About - CaspSites

General Information

CaspSites is a collection of experimentally identified human caspase substrates observed in apoptotic cells and lysates. Stored data was collected from a number of published studies using N-terminal labeling techniques to identify proteolytic events in human cell lines (see below for data set breakdown, their overlap, and references).

Search Capabilities

Users can perform queries on CaspSites content by entering lists of proteins and selecting P4-P4' site residues to search custom-selected datasets. Common substrates between multiple caspase data sets can be found by pressing the logical operator toggle found on the search page. A workflow diagram of the operations performed and layout of CaspSites can be seen on the right.

Distribution of Caspase Targets (P1=D) Stored in CaspSites

Data Set	# Cleavage Sites	# Substrates
Caspase-1	78	73
Caspase-2	300	253
Caspase-3	1197	957
Caspase-4	2	2
Caspase-5	1	1
Caspase-6	1120	871
Caspase-7	211	190
Caspase-8	65	62
Caspase-9	137	124
DegraBase	1706	1268
Molecule 1541	439	368
Fas	55	47
JG98	350	293
Apoptotic Cells	1902	1373
Lysates (Caspase 1-9)	2028	1456

Protease Web - Overlap of Cleavage Sites Between Datasets - link to interactive map (takes ~3-5 min to load; must stay on page)

Programmer Access to CaspSites (MongoDB API Drivers)

If you would like to directly access data stored in CaspSites (read-only), click here for more information about the programming languages and APIs supported by MongoDB.
- Please use the following access information (case-sensitive) - contact us to learn more about database structure:

Username	Password	Connection String	Database Name	Collection Name
CaspSitesGuest	caspase	mongodb+srv://<username>:<password>@julienlab.fibou.mongodb.net/?retryWrites=true&w=majority	CaspSitesGuest	CaspSitesData

Data Sources - Reverse (Caspase Treated Lysates)

Caspase-1, -4, -5 (Agard et al., 2010) — /
Caspase-2, -3, -7 (Wejda et al., 2012) – /
Caspase-3, -7, -8, -9 (Agard et al., 2012) – /
Caspase-2, -6 (Julien et al., 2016) — /
Caspase-7 (Hill et al., 2016) — /
Caspase-3, -9 (Araya et al., 2021) — /

Data Sources - Forward (Apoptotic Cells)

Fas (Van Damme et al., 2005) — /
DegraBase (Crawford et al., 2013) — /
Molecule 1541 (Julien et al., 2014) — /
JG-98 (Srinivasan et al., 2018) — /

Reference - learn more about database assembly and functionality:

Wang H. and Julien O. (2023) "CaspSites: A Database and Web Application for Experimentally Observed Human Caspase Substrates Using N-Terminomics"
J. Prot. Res. 22:454-461 /

Created by Henry Wang @ Julien Lab, Department of Biochemistry